QuantPrime is an intuitive and user-friendly, fully automated tool for primer pair design in small-
to large-scale real-time reverse transcription qPCR (also known as realtime qRT-PCR or RT-qPCR) analyses. QuantPrime can be used on the website or on a local computer
(contact us for getting a copy); it offers design and specificity
checking with highly customizable parameters and is ready to use with most publicly available eukaryotic
transcriptomes, including all higher eukaryote model organisms and important plant crops, while benefiting
from exon-intron border and splice variant information in available genome annotations. Experimental results
with the model plant Arabidopsis thaliana, the crop Hordeum vulgare (barley) and the model green alga
Chlamydomonas reinhardtii show success rates of designed primer pairs exceeding 96 %.
The QuantPrime service was created by Samuel Arvidsson - initially
at the University of Potsdam (supported by EU contract MRTN-CT-2006-035833), and is maintained by Jürgen Gremmels at the Max Planck Institute of Molecular Plant Physiology. The graphical design on the web site was created by Dr. Mirosław Kwaśniewski,
who also helped out with the design of the program together with Dr. Diego Mauricio Riaño-Pachón. The work was supervised by
Prof. Dr. Bernd Müller-Röber.
We kindly ask QuantPrime users for citation when primers are used in published works. Please cite as follows:
The online version of QuantPrime requires a modern web browser complying with the W3C HTML 4.01 and CSS 2.1 standards,